Construction and validation of a Bovine Innate Immune Microarray

BMC Genomics

Table 4 Summary of bovine and S. aureus control elements

Control Element Name	Reference Accession Number³	Start Position (bp)	Finish Position (bp)
β-actin 200	AY141970	1675	1804
β-actin 500¹	AY141970	1479	1804
β-actin 750	AY141970	1177	1804
β-actin 1000	AY141970	914	1804
β-actin 1500	AY141970	576	1804
β-actin 2000	AY141970	25	1804
β-actin 3'-region²	AY141970	1273	1822
β-actin Mid-region²	AY141970	784	1273
β-actin 5'-region²	AY141970	49	575
GAPDH 200	TC289232	1111	1347
GAPDH 580¹	TC289232	771	1347
GAPDH 750	TC289232	575	1347
GAPDH 1350	TC289232	1	1347
GAPDH 3'-region²	TC289232	409	876
GAPDH 5'-region²	TC289232	59	429
S. aureus GAPDH ²	NC_002745	832860	833305
S. aureus 30S ribosomal protein ²	NC_002745	1513891	1514573

¹ Denotes elements printed in a dynamic range of 25, 50 100, 200 and 400 pg per spot. All other elements were printed at 200 pg per spot.
² Control elements not available in cDNA libraries were amplified using gene specific primers. Primer sequences are listed in Additional file 2.
³ Genbank sequence (AY or NC) or TIGR cluster (TC).

ISSN: 1471-2164