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Table 4 Summary of bovine and S. aureus control elements

From: Construction and validation of a Bovine Innate Immune Microarray

Control Element Name Reference Accession Number3 Start Position (bp) Finish Position (bp)
β-actin 200 AY141970 1675 1804
β-actin 5001 AY141970 1479 1804
β-actin 750 AY141970 1177 1804
β-actin 1000 AY141970 914 1804
β-actin 1500 AY141970 576 1804
β-actin 2000 AY141970 25 1804
β-actin 3'-region2 AY141970 1273 1822
β-actin Mid-region2 AY141970 784 1273
β-actin 5'-region2 AY141970 49 575
GAPDH 200 TC289232 1111 1347
GAPDH 5801 TC289232 771 1347
GAPDH 750 TC289232 575 1347
GAPDH 1350 TC289232 1 1347
GAPDH 3'-region2 TC289232 409 876
GAPDH 5'-region2 TC289232 59 429
S. aureus GAPDH 2 NC_002745 832860 833305
S. aureus 30S ribosomal protein 2 NC_002745 1513891 1514573
  1. 1 Denotes elements printed in a dynamic range of 25, 50 100, 200 and 400 pg per spot. All other elements were printed at 200 pg per spot.
  2. 2 Control elements not available in cDNA libraries were amplified using gene specific primers. Primer sequences are listed in Additional file 2.
  3. 3 Genbank sequence (AY or NC) or TIGR cluster (TC).