Figure 3
Total RNA and amplified RNA yields of equal volumes of microdissected tumor epithelium and stroma. The average yield of total RNA (left axis; closed symbols) isolated from tumor epithelium (triangles; mean 50 ng/mm2 of 10 μm thick sections), was higher than total RNA isolated from stroma (circles; mean 14 ng/mm2; p = 0.001). After two rounds of amplification, a higher difference was observed between yields of RNA (right axis; open symbols) of microdissected tumor epithelium (mean 33 μg/mm2) and stroma (mean 4 μg/mm2; p < 0.0001).