Table 2 Effect of the second round of amplification. Pearson correlation coefficients evaluating the effect of the second round of amplification on the gene expression profiles. Twice-amplified macrodissected samples were compared to the corresponding once-amplified macrodissected samples. Correlation of duplicate amplification and labeling experiments are presented as well. In case of repeated experiments, Pearson correlation coefficients were calculated for each experiment and averaged.
Sample | Correlation Coefficient | p-value |
|---|---|---|
2 | 0.24 | <0.001 |
5 | 0.24 | <0.001 |
8 | 0.00 | 0.961 |
10 | 0.19 | <0.001 |
11 | 0.17 | <0.001 |
14 | 0.15 | <0.001 |
labelling | 0.95 | <0.001 |
amplification | 0.81 | <0.001 |