Skip to main content

Table 1 Sequencing and quality parameters of white spruce cDNA libraries. Quality reads had a Phred score above 20 over at least 100 bp after vector trimming.

From: Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters

Libraries, treatments and tissues Number of reads Library quality Sequence quality
  3' 5' % Empty % >1.6 Kb Nb of quality reads % Quality reads Average length of quality reads (nt)
Male strobili development sequence 1,536 1,536 4 19 2,589 84 527
Female cones development sequence 1,536 1,536 15 9 2,324 76 500
Vegetative buds development sequence 1,536 0 5 15 1,062 69 560
Secondary xylem – mature trees 4,608 4,608 10 27 7,735 84 600
Cambium, phloem – mature trees 4,608 3,072 2 8 6,705 87 635
Secondary xylem – girdled seedlings 3,072 0 9 24 1,053 69 556
Cambium to bark – girdled seedlings 1,536 1,536 NA NA 937 31 577
Elongating root tips – saplings 1,536 1,536 6 19 1,053 69 395
Primary, secondary shoots-N treatments 3,072 1,536 16 50 3,031 66 736
Immature somatic embryos 3,072 0 4 44 2,220 72 692
Clean roots systems – N treatments 1,536 0 7 37 858 56 659
Clean roots systems – P treatments 3,072 1,536 15 19 3,776 82 705
Clean roots systems – Diurnal cycle 6,144 4,608 16 33 8,601 80 757
Root secondary xylem – mature trees 3,072 0 7 8 1,532 50 598
Annual flush shoots diurnal cycle – trees 4,608 3,072 11 10 5,164 67 658
Needles – N fertilization treatments 1,536 0 15 20 461 30 686
Total 46,848 24,576    49,101