Skip to main content

Table 1 Sequencing and quality parameters of white spruce cDNA libraries. Quality reads had a Phred score above 20 over at least 100 bp after vector trimming.

From: Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters

Libraries, treatments and tissues

Number of reads

Library quality

Sequence quality

 

3'

5'

% Empty

% >1.6 Kb

Nb of quality reads

% Quality reads

Average length of quality reads (nt)

Male strobili development sequence

1,536

1,536

4

19

2,589

84

527

Female cones development sequence

1,536

1,536

15

9

2,324

76

500

Vegetative buds development sequence

1,536

0

5

15

1,062

69

560

Secondary xylem – mature trees

4,608

4,608

10

27

7,735

84

600

Cambium, phloem – mature trees

4,608

3,072

2

8

6,705

87

635

Secondary xylem – girdled seedlings

3,072

0

9

24

1,053

69

556

Cambium to bark – girdled seedlings

1,536

1,536

NA

NA

937

31

577

Elongating root tips – saplings

1,536

1,536

6

19

1,053

69

395

Primary, secondary shoots-N treatments

3,072

1,536

16

50

3,031

66

736

Immature somatic embryos

3,072

0

4

44

2,220

72

692

Clean roots systems – N treatments

1,536

0

7

37

858

56

659

Clean roots systems – P treatments

3,072

1,536

15

19

3,776

82

705

Clean roots systems – Diurnal cycle

6,144

4,608

16

33

8,601

80

757

Root secondary xylem – mature trees

3,072

0

7

8

1,532

50

598

Annual flush shoots diurnal cycle – trees

4,608

3,072

11

10

5,164

67

658

Needles – N fertilization treatments

1,536

0

15

20

461

30

686

Total

46,848

24,576

  

49,101

 Â