Effect of A23187 on B lymphoblasts cell cycle. The B lymphoblasts cultured in the absence or presence of 200 nM A23187 for 48 hours were stained with PI and analyzed for the proportion of cells in the different phases of the cycle by flow cytometry as described in Methods. Two independent experiments were performed with each one of five cell lines (two Scott B lymphoblasts independently immortalized from the patient's lymphocytes, one daughter's and two control cell lines from unrelated individuals). Data are means ± SD from the four independent experiments for Scott and control B lymphoblasts respectively and the two experiments for daughter's cells. For a given phase of the cell cycle and a cell type, P reflects the statistical differences between the percent of cells in the presence versus absence of A23187. * P = 0.0038, # P = 0.0016, † P = 0.0344, ★ P = 0.004, ◆ P = 0.0134.