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Figure 2 | BMC Genomics

Figure 2

From: Limitations of mRNA amplification from small-size cell samples

Figure 2

a and b. Correlation of transcript concentration estimates. Using the TransCount method, we obtained for each gene the distribution of the correlation coefficients between the reference sample transcript concentrations and the 1000 (250) cell sample transcript concentrations. Summary values for a certain gene in the (mean) 1000 cell samples versus the (mean) reference samples, are plotted against the estimated concentration for the (mean) reference sample in Fig. 2a. The black solid line is the median correlation coefficient values. The blue and green dashed lines are the 2.5% and 97.5% quantiles, respectively. The vertical dashed black line is the reliability threshold 121, i.e. the value for which the probability of positive correlation is at least 0.99. Similarly, information about the distribution for the (mean) 250 cell sample and (mean) reference is summarized in Fig 2b. In this case, the reliability threshold is 1806. The number of genes per concentration is shown below each respective plot. For a certain concentration c, the number of genes is counted from the interval

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