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Table 1 Estimates of input RNA quantities and resulting yield for 10 000 cells (reference) and 1000 and 250 cells (test), respectively. Calculation of the average fold yield of aRNA after two rounds of amplification are based on the assumption that 2% of total RNA represents mRNA.

From: Limitations of mRNA amplification from small-size cell samples

Cell sample size

~Total RNA

~mRNA (2% of total RNA)

Synthetic mRNA

Sum mRNA

Average aRNA yield

Average amplification factor

10 000

~115 ng

~2.3 ng

3.0 ng

5.3 ng

54 ÎĽ g

1.02 Ă— 104

1000

~11.5 ng

~0.23 ng

0.3 ng

0.53 ng

3.66 ÎĽ g

0.69 Ă— 104

250

~2.88 ng

~0.058 ng

0.075 ng

0.133 ng

1.18 ÎĽ g

0.89 Ă— 104