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Table 3 Description of pools and experiments in microarray testing

From: Proposed methods for testing and selecting the ERCC external RNA controls

Pool External RNA Clones Background RNA No. of Arrays
0 1 to 144 none 0
1 1 to 48 pre-labeled none 3
2 49 to 96 pre-labeled none 3
3 97 to 144 pre-labeled none 3
4 1 to 144pre-labeled none 0
5 1 to 72 (high conc.) 73 to 144 (low conc.) human 3
6 1 to 72 (low conc.) 73 to 144 (high conc.) human 3
7* 1 to 96 (diff. conc.) human 12
8* 1 to 96 human 12
9* 1 to 96 human 12
10* 1 to 96 human 12
11 1 to 96 human 0
12 1 to 96 human 3
13 1 to 96 human 3
14 1 to 96 human 3
   Total 75
  1. *Pools 7–10 may be further diluted to expand the concentration range tested.
  2. Array count is per one-color platform and does not include background RNA negative control samples.
  3. Pools 0 and 11 will also be used in QRT-PCR assays.