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Table 3 Description of pools and experiments in microarray testing

From: Proposed methods for testing and selecting the ERCC external RNA controls

Pool

External RNA Clones

Background RNA

No. of Arrays

0

1 to 144

none

0

1

1 to 48 pre-labeled

none

3

2

49 to 96 pre-labeled

none

3

3

97 to 144 pre-labeled

none

3

4

1 to 144pre-labeled

none

0

5

1 to 72 (high conc.) 73 to 144 (low conc.)

human

3

6

1 to 72 (low conc.) 73 to 144 (high conc.)

human

3

7*

1 to 96 (diff. conc.)

human

12

8*

1 to 96

human

12

9*

1 to 96

human

12

10*

1 to 96

human

12

11

1 to 96

human

0

12

1 to 96

human

3

13

1 to 96

human

3

14

1 to 96

human

3

  

Total

75

  1. *Pools 7–10 may be further diluted to expand the concentration range tested.
  2. Array count is per one-color platform and does not include background RNA negative control samples.
  3. Pools 0 and 11 will also be used in QRT-PCR assays.