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Table 6 Concentration of controls in dilution pools for expanded range experiments

From: Proposed methods for testing and selecting the ERCC external RNA controls

Pool

Conc. A Dil. 2

Conc. B Dil. 2

Conc. C Dil. 2

Conc. D Dil. 2

Conc. A Dil. 3

Conc. B Dil. 3

Conc. C Dil. 3

Conc. D Dil. 3

Conc. A Dil. 4

Conc. B Dil. 4

Conc. C Dil. 4

Conc. D Dil. 4

Pool 7

250

2

10

50

500

4

20

100

5,000

40

200

1,000

Pool 8

50

250

2

10

100

500

4

20

1,000

5,000

40

200

Pool 9

10

50

250

2

20

100

500

4

200

1,000

5,000

40

Pool 10

2

10

50

250

4

20

100

500

40

200

1,000

5,000

  1. Concentration is given as mass ratios, so that "250" represents 1:250,000 or 1 ng of RNA transcript per 250 ng of background RNA, where the spike amount is adjusted for its length.
  2. Concentrations of the initial stock, "Dilution 1" pools are shown in Table 4.