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Table 6 Concentration of controls in dilution pools for expanded range experiments

From: Proposed methods for testing and selecting the ERCC external RNA controls

Pool Conc. A Dil. 2 Conc. B Dil. 2 Conc. C Dil. 2 Conc. D Dil. 2 Conc. A Dil. 3 Conc. B Dil. 3 Conc. C Dil. 3 Conc. D Dil. 3 Conc. A Dil. 4 Conc. B Dil. 4 Conc. C Dil. 4 Conc. D Dil. 4
Pool 7 250 2 10 50 500 4 20 100 5,000 40 200 1,000
Pool 8 50 250 2 10 100 500 4 20 1,000 5,000 40 200
Pool 9 10 50 250 2 20 100 500 4 200 1,000 5,000 40
Pool 10 2 10 50 250 4 20 100 500 40 200 1,000 5,000
  1. Concentration is given as mass ratios, so that "250" represents 1:250,000 or 1 ng of RNA transcript per 250 ng of background RNA, where the spike amount is adjusted for its length.
  2. Concentrations of the initial stock, "Dilution 1" pools are shown in Table 4.