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Table 7 Expected red:green ratios in two-color hybridizations

From: Proposed methods for testing and selecting the ERCC external RNA controls

Array Pool in Green Channel Pool in Red Channel Group A Ratio Group B Ratio Group C Ratio Group D Ratio
1 P7-D1 P10-D1 0.008 5 5 5
2 P8-D1 P9-D1 0.2 0.2 125 0.2
3 P9-D1 P8-D1 5 5 0.008 5
4 P10-D1 P7-D1 125 0.2 0.2 0.2
5 P7-D2 P8-D2 0.2 125 0.2 0.2
6 P8-D2 P7-D2 5 0.008 5 5
7 P9-D2 P10-D2 0.2 0.2 0.2 125
8 P10-D2 P9-D2 5 5 5 0.008
9 P7-D3 P9-D3 0.04 25 25 0.04
10 P8-D3 P10-D3 0.04 0.04 25 25
11 P9-D3 P7-D3 25 0.04 0.04 25
12 P10-D3 P8-D3 25 25 0.04 0.04
13* P7-D4 P7-D4 1 1 1 1
14* P8-D4 P8-D4 1 1 1 1
15* P9-D4 P9-D4 1 1 1 1
16* P10-D4 P10-D4 1 1 1 1
  1. *Self-to-self hybridizations
  2. Pools in each channel are labeled based on their pool and dilution numbers in Table 6.
  3. Groups are sets of transcripts at the same concentration as defined in Table 4.