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Table 1 Proportion of reporter transcripts encoding clones in the subtracted libraries

From: SSH adequacy to preimplantation mammalian development: Scarce specific transcripts cloning despite irregular normalisation

 

Library

Initial abundance (%)

Tester specific (bold)

Tester/Driver Common (standard)

Hybridised/analysed clones

Proportion in subtracted library (%)

Rare transcripts

rab1

#

#

#

 

bov1

0,001

13/4608

0,28

 

rab2

0,0005

3/2683

0,11

 

rab3

0,0005

14/4608

0,3

Moderate transcripts

rab1

0,005

5/768

0,65

  

0,005

0/768

-

 

bov1

0,005

24/4608

0,52

  

0,005

3/4608

0,06

 

rab2

0,002

22/2683

0,82

  

0,002

0/2683

-

 

rab3

0,002

11/4608

0,24

  

0,002

2/4608

0,043

Abundant transcripts

rab1

0,05

82/768

10,7

  

0,05

10/768

1,3

 

bov1

0,05

195/4608

4,2

  

0,05

4/4608

0,086

 

rab2

0,01

147/2683

5,48

  

0,01

0/2683

-

 

rab3

0,01

256/4608

5,55

  

0,01

25/4608

0,54

  1. #: not introduced in library
  2. -: no clone detected among the screened clones