Reversed-Phase HPLC purification of granulin-A and granulin-1 from extracts of two carp spleens. Panel A: HPLC fractions derived from an extract of two carp spleens and enriched in granulin-1 immunoreactivity were further purified by HPLC using 0.13% heptafluorobutyric acid as counterion. Two major components were identified, a peptide sharing 58% sequence conservation with human granulin-A (peak A) and granulin-1 (peak 1), each indicated by arrows. Panel B: Sequence comparison of carp granulin peptides with their respective candidate orthologues deduced from cloned zebrafish cDNA sequences (this study), and human granulin-A. Numbers correspond to amino acid position. Characteristic cysteines are shown in bold.