Orientation of the LMP2A transgene array. (A) LMP2A transgene structure. Restriction enzyme sites and primer location and orientation are shown. (B) LMP2A transgene amplification in TgPWS brain (Br) and liver (Li) (lanes 2,4) using primers RN1316 and RN1317 compared with the WT controls (lanes 3,5). (C-D) The use of (C) RN1316 or (D) RN1317 alone fails to generate PCR products in TgPWS and WT brain and liver.