Skip to main content

Table 2 BAC end sequences of the positive clones mapped on the C57BL/6J genome

From: Construction and characterization of a genomic BAC library for the Mus m. musculus mouse subspecies (PWD/Ph inbred strain)

Gene

Probe position

BAC clone/primer

BES position

Strand

Insert size (bp)

     

mapped

PFGE

Mash

chr7:130,672,998

327-5I/T7

chr7:130,587,753

+

132,317

145,000

  

327-5I/SP6

chr7:130,720,069

-

  

Ads

chr12:35,152,780

262-11G/SP6

multiple hits

 

n.d.

105,000

  

262-11G/T7

chr12:35,192,443

-

  
  

266-2E/SP6

chr12:35,090,782

+

109,399

115,000

  

266-2E/T7

chr12:35,200,180

-

  

Igf2r

chr17:12,150,509

279-5I/SP6

chr17:12,057,702

+

125,000

120,000

  

279-5I/T7

chr17:12,182,701

-

  
  

282-5D/T7

chr17:12,028,446

+

144,525

145,000

  

282-5D/SP6

chr17:12,172,970

-

  
  

245-11P/SP6

chr17:12,000,500

+

186,822

195,000

  

245-11P/T7

chr17:12,187,321

-

  

Tbp

chr17:14,324,440

297-2I/T7

chr17:14,291,556

+

147,925

145,000

  

297-2I/SP6

chr17:14,439,480

-

  

Usp26

chrX:44,101,113

293-21P/T7

chrX:44,086,068

+

184,994

190,000

  

293-21P/SP6

chrX:44,271,061

-

  

Xist

chrX:94,885,652

269-9H/SP6

chrX:94,817,775

+

150,314

160,000

  

269-9H/T7

chrX:94,968,088

-

  
  

255-3L/T7

chrX:94,756,622

+

134,724

145,000

  

255-3L/SP6

chrX:94,891,345

-

  
  

271-12L/SP6

chrX:94,854,496

+

131,966

140,000

  

271-12L/T7

chrX:94,986,461

-

  

Tex13

chrX:130,816,302

257-22B/SP6

chrX:130,761,904

+

124,211

120,000

  

257-22B/T7

chrX:130,886,114

-

  
  

322-4J/SP6

chrX:130,761,839

+

117,691

110,000

  

322-4J/T7

chrX:130,879,529

-

  
  1. BAC ends (BESs) of the positive clones identified on the membranes no. 6 and no. 7 were sequenced, mapped and aligned to the mouse genome (mm5 assembly, UCSC). The coordinates on the mouse genome are shown for the probes as well as for the ends of genomic inserts. An average insert size 140.8 kb was calculated from mapped positions and 141.2 kb was determined by pulsed-field gel electrophoresis (PFGE).