Figure 1From: Efficient gene-driven germ-line point mutagenesis of C57BL/6J miceGene-driven ENU-induced mutagenesis of any mouse gene using the Cryopreserved Mutant Mouse Bank (CMMB). Flowchart showing: generation of the CMMB, PCR amplification of selected genes from the DNA and cDNA templates, mutation screening of PCR products by TGCE and DNA sequencing, and recovery of mutant mice from cryopreserved sperm by ICSI (see text for details).Back to article page