Table 1 Statistics on shotgun sequencing of the wild rat-derived genomic library

picked colonies

1632

readable sequence reads/sequenced bases

2975/814,440

uniquely mapped (BLAT) reads/bases

2545/768,683

ambiguous positions

4902

strong candidates after manual inspection

868 (746 SNPs + 122 indels)

successfully read/amplicons designed*

416/451 (~1.65 candidate SNP/amplicon) (92.2%)

amplified bases per strain or wild individual

118,971