Multiple alignment of the HET-s/LopB protein family. The first line in the alignment shows the prediction of secondary structure content: h for helical, e for extended, c for coiled. Residues conserved among several proteins are marked with gray shading. The polymorphic positions in HET-s and HET-S proteins are shown with red shading. Proteins are listed under their unique GenBank identifiers (first left column). Species are indicated in the second from left column: Pans, Podospora anserina; Lmac, Leptosphaeria maculans; Mgri, Magnaporthe grisea; Ncra, Neurospora crassa; Anid, Aspergillus nidulans, Gzea, Gibberella zeae. Yellow shading indicates uncharged amino acids (A, I, L, V, M, F, Y, or W). Conserved small residues (G, A, or S) are shown in green. Charged residues (D, E, K, R, N, or Q) are shown in blue. The residues corresponding to the proteinase K-resistant amyloid core in P. anserina Het-s are highlighted in purple and underscored.