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Figure 2 | BMC Genomics

Figure 2

From: Comparison of gene expression signatures of diamide, H2O2 and menadione exposed Aspergillus nidulans cultures – linking genome-wide transcriptional changes to cellular physiology

Figure 2

Dose-dependence and time-course of transcriptional effects of diamide, H2O2 and menadione on selected genes. Part A. Changes in the transcription of aoxA, gstA, sodA, sconC and a homologue of the Ca2+-calmodulin-dependent serine-threonine-protein kinase of S. pombe (ORF ID: AN4483.2) shown by Northern blot hybridization. Parts B-D. Graphical representation of optical density ratios of autoradiographic bands visualized on Northern blots in parallel stress-exposure and control experiments for gstA, sodA and the A. nidulans homologue of S. pombe Ca2+-calmodulin-dependent serine-threonine-protein kinase, respectively. By definition, MNorthern = log2(optical densitystress-exposed*optical densitycontrol-1). Parts E-G. Time-courses of the transcriptional changes recorded in DNA microarray experiments for gstA (ORF ID: AN4905.2, OSU contig ID: contig2000Sep131300_1307), sodA (ORF ID: AN0241.2, OSU contig ID: contig2000Sep131300_575) and the A. nidulans homologue of S. pombe Ca2+-calmodulin-dependent serine-threonine-protein kinase (ORF ID: AN4483.2, OSU contig ID: contig2000Sep131300_1404), respectively. Symbols ◆, ▲ and ■ stand for 1.8 mM diamide, 75 mM H2O2 and 0.8 mM menadione treatments, respectively.

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BMC Genomics

ISSN: 1471-2164

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