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Figure 5 | BMC Genomics

Figure 5

From: High-throughput sequencing: a failure mode analysis

Figure 5

Process failure mode distribution (A) Process-related failure mode distribution. Most of the process-related failed reads have a Q20 < 100 with the primary mode of failure being "Low signal, no DNA," or no template DNA in the sequencing reaction (confirmed by agarose gel electrophoresis). (B) Template-related failure mode distribution. Most of the template-related failed reads are a result of "Poly A Tail," or 3' poly A stretch that leads to slippage in sequence data. Reads with a "Low signal 5', 3' template characteristic" failure mode contribute to the largest proportion of data with read lengths Q20 < 100.

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