Table 2 Breakdown of "Low signal strength" reads Reads from the preliminary "Low signal strength" failure mode category (310 reads) are further categorized into finer failure mode classifications. Failed reads from each unique clone are grouped together where possible (excluding reads that do not confirm presence of DNA on evaluation agarose gel) to determine mode of failure.
Groupings of failed reads | No. of occurrences | Process associated failures (No. of reads) | Template associated failures (No. of reads) | Failure mode |
|---|---|---|---|---|
F1/F2/R1/R2 | 4 | 0 | 16 | Low signal, 5' and 3' template characteristic |
F1/F2/R1 | 1 | 1 | 2 | 1. Low signal, DNA lost during precipitation 2. Low signal, 5' template characteristic |
F2/R1/R2 | 1 | 1 | 2 | 1. Low signal, DNA lost during precipitation 2. Low signal, 3' template characteristic |
R1/R2 | 43 | 0 | 86 | Low signal, 3' template characteristic |
F1/F2 | 5 | 0 | 10 | Low signal, 5' template characteristic |
F1/R1 | 1 | 2 | 0 | Low signal, DNA lost during precipitation |
No available clone pairing (singleton) | 44 | 44 | 0 | Low signal, DNA lost during precipitation |
No DNA in agarose gel | 146 | 146 | 0 | Low signal, no DNA |