Table 2 Yield and transcript length of amplified RNA produced by three amplification methods. Values in parentheses represent p-values from t-test comparing the value against that of the study protocol.
From: A robust method for the amplification of RNA in the sense orientation
Study Protocol | RiboAmp Protocol (p-value vs. Study Protocol) | BDSMart Protocol (p-value vs. Study Protocol) | |
|---|---|---|---|
Mean yield from 4 μg total RNA ( | 26.29 | 71.56 (<0.04) | 4.87 (<<0.0001) |
Median amplified RNA length (nt) | 794 | 507 (<0.003) | 764 (0.88) |
, μg)