Table 3 Statistical analysis of microarray data generated by comparing hybridizations of amplified RNA samples to identical hybridizations of unamplified (total RNA) samples. Values in parentheses represent P-values from t-test comparing the value to that of the study protocol.
From: A robust method for the amplification of RNA in the sense orientation
Study Protocol | RiboAmp Protocol (p-value vs. Study Protocol) | BDSmart Protocol (p-value vs. Study Protocol) | |
|---|---|---|---|
Mean Correlation Coefficient ( | 0.8009 | 0.7202 (<0.01) | 0.7679 (0.23) |
Mean % of elements with absolute difference of ± 2 log2 units | 0.34 | 1.34 (<0.02) | 0.60 (0.56) |
Mean % of elements with absolute difference of ± 1.5 log2 units | 1.31 | 4.05 (<0.05) | 1.63 (0.67) |
Mean % of elements with absolute difference of ± 2 log2 units | 5.44 | 12.54 (<0.02) | 6.52 (0.61) |
Mean % of amplified array elements within ± 1 log2 unit | 94.56 | 87.46 (<0.02) | 93.48 (0.61) |
)