Table 4 Primers used for qPCR.
From: Gene expression in the brain and kidney of rainbow trout in response to handling stress
Gene | Sequence |
|---|---|
GRB2-related adaptor protein 2 | Forward 5'-GCCAGAGCACCCCAGGAGAT-3' Reverse 5'-GGCTGAGAGGATGGGGCTGA-3' |
Collagenase type IV | Forward 5'-AACATCAGAAACGCCCTCAT-3' Reverse 5'-TGGTGGTAGTGGTAGTGGAC-3' |
Troponin T | Forward 5'-TGGGAAGAAGGAAACTGAGA-3' Reverse 5'-CTCTTACGCAGGGTTGTGAC-3' |
40S ribosomal protein S12 | Forward 5'-AGACCGCACTCATCCACGAC-3' Reverse 5'-CCACTTTACGGGGTTTTCCT-3' |
EST1 | Forward 5'-CGGAGAAGGAGAACCCACAG-3' Reverse 5'-CCCTCAAACAAGCAAAGTG-3' |
EST2 | Forward 5'-GCAAATGACAGCCCTCTTAG-3' Reverse 5'-AGCAGGTTTTCATCAAGGA-3' |