Figure 4

Expression of Psg mRNAs during placental development. Total RNA (1 μg) from day 10.5, 12.5, 15.5 and 17.5 BALB/c placentae was reverse transcribed using an oligo (dT) oligonucleotide (reverse PCR primer). After addition of the degenerate Psg-all oligonucleotide (forward PCR primer), which anneals to the cDNA of all known members of the mouse Psg family, Psg cDNAs were amplified by PCR (see schematic diagram depicting generalised mouse Psg cDNA amplification). Aliquots were size-separated by agarose gel electrophoresis. a, PCR products were visualised by ethidium bromide staining. b-o, the amplification products were blotted onto nylon membranes and individual blots were hybridised with single gene-specific 32P-labelled oligonucleotides from the N1 domain regions (Table 2). The location of the primers used for amplification of the Psg cDNAs and the region from which the sequences of the gene-specific oligonucleotides were derived are shown together with a schematic representation of mouse Psg mRNA. The 5'- and 3'-untranslated regions are shown as bold lines. L, leader; N1-N3, IgV-like domains; A, IgC-like domain.