Figure 6

Physical map of mouse Psg gene locus. A. The order of the Psg genes was inferred from the presence of the various genes on overlapping cosmid, BAC and YAC clones. The position of Psgs represented by filled boxes is unequivocal, whereas the position of those represented by open boxes is ambiguous. Arrows between pairs of genes indicate that their order remains unresolved. The distances between individual genes are not shown to scale. Chimeric YACs mapping to separate chromosomes are indicated by stippled and solid lines. The solid lines correspond to chromosome 7 regions containing the Psg genes indicated above. The locations of the non-chromosome 7 regions are not known. Only the sizes of non-chimeric YACs have been determined and are shown (size bar corresponds to 100 kb). The centromere (cen) / telomere (ter) order and the relative orientation of the two Psg gene subclusters were resolved by FISH mapping. B. Two-colour FISH prophase mapping of relative orientation of the two Psg gene subclusters using mouse m5S cells and C57BL/6CrSlc mouse lymphocytes. (i) FISH pattern representative of 38 experiments where BAC 310D2 in subcluster 1, labelled with rhodamine (R), is centromeric to BAC 600E2 from subcluster 2, labelled with fluorosceine (F). (ii) FISH pattern representative of 38 experiments where BAC 310D2 in subcluster 1, labelled with rhodamine, is centromeric to YAC F10104 from subcluster 2, labelled with fluorosceine. (iii) Orientation of subcluster 2 determined by relative positions of BAC 572D4, labelled with rhodamine, which is telomeric to YAC F10104, labelled with fluorosceine.