Tissue distribution of mMagT1 protein. A. Western blots of membrane proteins from tissue extracts. Extracts were prepared from tissues as described under “Experimental Procedures”. MagT1 bands were probed with anti-MagT1antibody. Molecular sizes are expressed in kDa of pre-stained standards are shown on the left of each of the representative blots. B, summary of 38 kDa MagT1 protein in 15 μg total protein from various mice tissues. Data were obtained from 3 different mice and are indicated as the mean ± SEM. C, specificity of anti-MagT1 antibody. The fractions isolated from normal and magnesium-depleted MDCT cells were blotted with MagT1 antibody and MagT1 antibody preadsorbed with excess antigen peptide. The signal was reduced to background levels when preadsorbed antibody was used indicating that the antibody was specific to MagT1.