Table 1 Number of BAC clones detected after hybridization and PCR verification of probes flanking microsatellites
GenBank accession no. | Mic.sat. clone name | No. of clones observed in secondary hybridisation | Verified by PCR | Positive PCR product on unassigned clones | Total no. of clones verified by PCR |
|---|---|---|---|---|---|
AF256671 | BHMS-175 | 28 | 28 | 7 | 35 |
AF256676 | BHMS-189 | 24 | 21 | 5 | 26 |
AF256678 | BHMS-201 | 9 | 9 | 4 | 13 |
AF256741 | BHMS-289 | 19 | 18 | 5 | 23 |
AF256698 | BHMS-304 | 19 | 18 | 6 | 24 |
AF256748 | BHMS-330 | 12 | 12 | 1 | 13 |
AF256746 | BHMS-349 | 23 | 23 | 4 | 27 |
AF256719 | BHMS-429 | 42 | 42 | 29 | 71 |
AF256693 | BHMS-278 | 8 | * | * | * |
AF256848 | BHMS-255 | 24 | # | # | # |
AF256750 | BHMS-337 | 21 | 5 | 29 | 34 |
AF256714 | BHMS-396 | 4 | 3 | 1 | 4 |
Total number of clones: | 233 | 179 | 91 | 270 | |
Average | 19.4 | 19.9 | 9.1 | 27.0 |