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Table 1 Number of BAC clones detected after hybridization and PCR verification of probes flanking microsatellites

From: A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects

GenBank accession no. Mic.sat. clone name No. of clones observed in secondary hybridisation Verified by PCR Positive PCR product on unassigned clones Total no. of clones verified by PCR
AF256671 BHMS-175 28 28 7 35
AF256676 BHMS-189 24 21 5 26
AF256678 BHMS-201 9 9 4 13
AF256741 BHMS-289 19 18 5 23
AF256698 BHMS-304 19 18 6 24
AF256748 BHMS-330 12 12 1 13
AF256746 BHMS-349 23 23 4 27
AF256719 BHMS-429 42 42 29 71
AF256693 BHMS-278 8 * * *
AF256848 BHMS-255 24 # # #
AF256750 BHMS-337 21 5 29 34
AF256714 BHMS-396 4 3 1 4
Total number of clones:   233 179 91 270
Average   19.4 19.9 9.1 27.0
  1. * PCR products was not observed from BAC template, and a weak smear was observed using genomic DNA
  2. # Weak smear was observed from BAC template and genomic DNA