Conversion of cDNA differential display results (DDRT-PCR) into quantitative transcription profiles

BMC Genomics

Table 1 Solitary cDNA fragments

Bands detected in treated sample but missing in the control
Relative intensity*	Number of bands
0 – 30%	3
30% – 300%	19
> 300%	10
Bands detected in the control but missing in treated sample
Relative intensity*	Number of bands
0 – 30%	3
30% – 300%	13
> 300%	6

*as compared to the average of densitometric values of all bands in the experiment DDRT-PCR bands in L. maculans cultures treated with Brassica napus secondary metabolites vs. untreated control were matched and their intensities compared (Fig. 5 and 6). For bands with no counterpart, the relative intensity as compared to the average intensity of all bands in the experiment was determined.

ISSN: 1471-2164