Figure 5

Unique combinations of genomic differences between different strains. Panel A: Selected portions of the averaged data shown in Karyoscope form in Figure 3 are shown here in color-block form. To generate this figure the averaged data for all four wine strains were sorted for the Montrachet strain from highest green values (greatest negative number) to highest red values (greatest postitive number) and visualized as a "clustergram" using Java TreeView (note that the data were sorted, but however, were not clustered). The topmost (most green) 25 genes are shown in the top panel; the lower two panels show the sorted data surrounding two of the genes (AYT1 in the middle panel, YPL257W in the lowest panel) whose copy number varies greatly on a strain-to-strain basis. Red asterisks mark the genes whose copy numbers vary in unique combinations between the different strains. Panel B shows a matrix of gene copy number changes for each of the 12 wine strain isolates, along with the drug response profiles of each strain isolate at the bottom. Within the copy number data portion, a dark red cell with a "++" symbol indicates a red:green log ratio greater than 0.8, light red with "+" indicates a ratio between 0.2 and 0.8, and gray with "+/-" indicates a ratio between 0.2 and -0.2. Likewise dark green with "--" indicates a red:green log ratio less than -0.8, light green with "-" indicates a ratio between -0.2 and -0.8.