Figure 6
Drug resistance phenotypes of wine strains. Halo assays were performed as described in the text to test the response of the wine strains to various drugs. Panels A and B show photographs of the halos; on the far left of each panel are shown the halo assays for the WT-diploid (FY1679, a S288C-based diploid, see Methods), against which all other strains were compared. CYH = 500 ng cycloheximide; CTZ = 5 μg clotrimazole; SMM = 20 μg sulfomethuron methyl. Panel A: strains are as described in Methods and Table 1; "WT-Dip" refers to FY1679. Panel B: Strains are as described in Methods; "WT-Dip" refers to FY1679; "YPL257 Δ" to GSY28; "ayt1 Δ" to ScAYT1Δ, "pdr5-" to JG436; and "WT-Hap" to S288C. Panel C: Graphical results of halo assays. Annular area (i.e., area of total halo minus area of disc) was calculated for each halo, then expressed as log2 of the ratio of the annular area to that of the WT-Dip (FY1679) annular area.