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Table 3 Mismatch probe signal intensities from sscDNA and cRNA hybridizations.

From: Increased DNA microarray hybridization specificity using sscDNA targets

Target

> 2× median*

> 4× median*

> 8× median*

sscDNA

14.1 ± 1.8%

5.0 ± 0.8%

2.0 ± 0.3%

cRNA

26.6 ± 1.4%

12.9 ± 0.9%

5.4 ± 0.6%

  1. The proportion of mismatch probes with intensities greater than 2, 4, or 8 times the median for all mismatch probes on the same array. Values are mean ± standard deviation for triplicate arrays hybridized with sscDNA or cRNA prepared from sUHR RNA. Each array had 201,800 mismatch probes.
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BMC Genomics

ISSN: 1471-2164

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