Figure 1

Pathway Voyager mapping procedure. The analysis and mapping procedure of PathwayVoyager is shown in a flowchart diagram. Manual selection of organisms and pathways present in the KEGG database, at the time of analysis, results in the retrieval of a specific set of protein sequences that are subsequently reformatted into a BlastP database. Protein query sequences are then used as templates for local BlastP analyses. Results are subjected to a user-defined threshold and subsequently parsed to retrieve tagged KEGG pathway maps. Pathway graphs and parsed BlastP results are stored as a flatfile database and can be displayed using the graphical browser. Opposite double arrows represent Internet-access using the KEGG API. Symbols used are according to general flowchart conventions.