Figure 2

Hybridization patterns of human and canine SEC61B gene to Affymetrix 203133_at probe set and alignment of 203133_at probe pairs to canine SEC61B gene sequence. Panels A and B represent screen shots of MAS 5.0 generated intensities for "perfect match" probes (grey columns) and "mismatched' (open columns), respectively. Panel C depicts CLUSTALW generated alignment of canine SEC61B gene (accession number L25052) and 11 "perfect match" probes of Affymetrix 203133_at probe set. Star signs represent matched base pairs and double-headed arrows show locations of 203133_at probes (the corresponding probe number attached at the 5' end of probes). Panel D represent human SEC61B gene hybridization patterns throughout 40 GeneChip arrays. The order numbers of 203133_at probes are shown on the left. GeneChips were grouped by tested tissues (8 tissue groups with 5 chips per group) and are labeled on the top of each profile. Probe pairs were divided into three groups: poorly performing (open blocks, (PM - MM) ≤ 20), moderately performing (grey blocks, 20 < (PM - MM) ≤ 200), and well performing (solid blocks, (PM - MM) > 200). Panel E represent canine SEC61B gene hybridization patterns throughout 40 GeneChip arrays.