Effects of human/canine genomic differences on probe performance and correlation of probe performance with sequence similarity. Only well performed during human/human hybridization probes were employed for analysis of the effects of genetic sequence differences between human and canine. Two pools of transcripts were probes with non-detectable hybridization signal in 95% cases (generated detectable signal during 0–2 hybridizations) and probes that generated detectable signal in 95% cases (38–40 hybridizations). Randomly selected probes from each pool were aligned to their canine counterparts and number of mismatched base pairs was identified. The difference in detected mismatches between these probe pools was evaluated using Mann-Whitney test with p < 0.05 used as a significance cut-off (insert).