Figure 6

Subcellular localization of recombinant fusion proteins in human cell lines. Human protein NP_219484 (C. elegans protein ZK795.3) was detected in transfected HeLa cells with goat anti-V5 conjugated with FITC and co-localized with human anti-fibrillarin subsequently detected by donkey anti-rabbit Cy3 (A). Nuclei were stained with DAPI. This protein displays both a nucleolar (top row) and nuclear speckle (bottom row) pattern (A). NP_848545 (C. elegans protein C17E4.3) was detected in HEK293 cells with mouse anti-V5 and goat anti-mouse Alexa 488 (B). Cells were costained with rabbit anti-Lamin and donkey anti-rabbit Cy3 illustrating a colocalization with the nuclear envelope (B). Partial ER and nuclear distributions were observed for NP_115683 by co-detection with rabbit anti-calreticulin in HeLa cells (C). AAP34400.1 was detected in HeLa cells at the plasma membrane by co-staining with Annexin II (left panel, D) and a partial colocalization with beta-actin was also detected (right panel, D). Scale bars represent 10 μm.