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Table 3 An illustration of concentrations of different species at a hybridization time of 24 hours. S0 = 10 pM, R0 = = 1 pM

From: An analysis of the use of genomic DNA as a universal reference in two channel DNA microarrays

kf-bound M-1 s-1

[B] pM

[B'] pM

[R] pM

[R'] pM

[R] [B'] pM2

[R'] [B] pM2

[B]/ [B']

[R]/ [R']

106

9.6 × 105

4.6 × 105

0.63

0.27

2.9 × 105

2.5 × 105

2.09

2.33

104

1.0 × 106

9.9 × 105

0.94

0.40

9.3 × 105

4.0 × 105

1.01

2.35

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BMC Genomics

ISSN: 1471-2164

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