Illustration of hyperspectral data cube and multivariate analysis results. (a) Three-dimensional hyperspectral data cube for an idealized two-component microarray containing emission only from labeled DNA within the printed spots. Each pixel in the x-y image plane contains an entire fluorescence emission spectrum from 550–900 nm. (b-c) Results of multivariate curve resolution on two-component sample hyperspectral data cube shown in A. (b) Pure component spectra identify "what" species are fluorescing in an image. (c) Corresponding component concentration maps show "where" and "how much" of each component in Fig. 1b is present.