Images illustrating dye separation. (a) Red/Green ratio image generated from Axon 4000B microarray scanner showing the red ring characteristic of apparent dye separation. (b) Cy5/Cy3 ratio image generated from HSS concentration maps showing uniform Cy5 and Cy3 distribution throughout the spot. (c-e) Individual component concentration maps resulting from the multivariate analysis of the HSS image data highlighting the difference in spot diameter of the brighter contaminant that results in the apparent dye separation when imaged with a filter-based microarray scanner. All fluorescence emissions in the hyperspectral images were excited simultaneously in a single scan. (c) Cy5 concentration map. (d) Cy3 concentration map. (e) Green contaminant concentration map.