Images illustrating background problems. All images are of same area on the microarray. (a) Red/Green ratio image generated from Axon 4000B microarray scanner. The green channel intensity dominates background and spot signal. (b-d) Individual component concentration maps extracted from multivariate analysis of HSS image data. All fluorescence emissions in the hyperspectral images were excited simultaneously in a single scan. (b) Glass concentration map. Note that the spots all appear slightly dimmer than the background. (c) Cy3 concentration map. (d) Cy5 concentration map.