Figure 4

Global comparison of gene expression changes in polyA mRNA and NRO RNA. A. At each time period indicated columns correspond to the values derived by subtracting the Z ratio of NRO RNA from the Z ratio of polyA mRNA for every gene. Equivalency between calculations varies around 0, data is aligned using a combined average index, and is displayed from left to right to represent the highest average positive Z ratio difference to the lowest average negative Z ratio difference. Columns in red correspond to genes exhibiting significant differences (Z ratio difference values greater or less than ± 1.5) in gene expression changes when comparing polyA mRNA and NRO RNA for each gene at each time point. B. Hierarchical clustering of all significant changes in gene expression in either NRO or polyA mRNA across the time course of activation. The median Z score data for each gene is individually normalized to its baseline value in for the sake of this comparison.