Primer design. To design primers, we aligned an An. gambiae putative histone H1 candidate XP_311486 (Panel A, top) with a histone H1 protein (Q07134; Panel A, bottom) from C. thummi. Boxed residues were chosen for design of primers, according to the An. gambiae nucleotide sequence. Panel B shows these primer residues aligned between the An. stephensi RPS6 tail (top), and the putative Anopheles gambiae histone H1 (bottom). Vertical bars designate identities.