Functional analysis and comparative genomics of expressed sequence tags from the lycophyte Selaginella moellendorffii

Table 4 Top 20 S. moellendorffii EST tBLASTX hits for A. thaliana ESTs that are not present within the P. patens EST database.

	Non-redundant EST	tBLASTX E-value	Best BLASTX Descriptor in A. thaliana	Accession Number
1	SmoC-1_01_H05	1E-107	expressed protein	REF:NP_194688
2	SmoC-1_02_C05	1E-99	oligopeptide transporter OPT family protein	REF:NP_192815
3	SmoC-1_01_L23	2E-99	putative Mg-protoporphyrin IX chelatase	REF:NP_196867
4	SmoC-1_05_G17	2E-99	putative caffeoyl-CoA 3-O-methyltransferase	REF:NP_195131
5	SmoC-1_05_K13	5E-90	chloroplast membrane protein (ALBINO3)	REF:NP_180446
6	SmoC-1_01_E02	1E-89	cullin family protein	REF:NP_567243
7	SmoC-1_05_G03	7E-87	putative UDP-galactose/UDP-glucose transporter	REF:NP_563949
8	SmoC-1_05_I19	6E-86	expressed protein	REF:NP_566060
9	SmoC-1_02_N15	9E-86	nicotinate phosphoribosyltransferase family protein	REF:NP_179923
10	SmoC-1_03_I01	7E-80	glycoside hydrolase family 77 protein	REF:NP_181616
11	SmoC-1_cn293	4E-77	amine oxidase family protein	REF:NP_181830
12	SmoC-1_03_C24	9E-73	uridylyltransferase-related protein	REF:NP_564010
13	SmoC-1_02_P14	5E-70	expressed protein	REF:NP_199542
14	SmoC-1_06_P21	2E-69	RNase L inhibitor protein-related	REF:NP_196569
15	SmoC-1_05_G10	3E-69	expressed protein	REF:NP_191746
16	SmoC-1_03_C14	1E-66	putative isoflavone reductase	REF:NP_565107
17	SmoC-1_03_N06	6E-65	transducin / WD-40 repeat family protein	REF:NP_190148
18	SmoC-1_06_M11	1E-63	dehydration stress-induced protein	GB:AAM62648
19	SmoC-1_06_B20	1E-60	putative membrane protein	REF:NP_849987
20	SmoC-1_05_O21	2E-60	paired amphipathic helix repeat-containing protein	REF:NP_186781

The tBLASTX E-value of an EST varies with its BLASTX E-value in a small range (e.g. SmoC-1_01_H05 has a tBLASTX E-value of 1E-107 against its homologous A. thaliana EST and a BLASTX E-value of 2E-94 against the translated full length A. thaliana cDNA.). The homology ranking was based on the tBLASTX E-value. The identities of ESTs were putatively described by the A. thaliana protein with the lowest E-value in the BLASTX analysis.

ISSN: 1471-2164