Skip to main content

Table 1 microRNA/target pairs predicted by MovingTargets using the following strict targeting criteria: minimum 3 target sites, maximum dG of microRNA/target hybridization of -15 kcal/mole at RT for each target site, minimum 7 out of 8 consecutive base pairs in microRNA 5' end, maximum 1 G:U base pair in microRNA 5' region. Transcription factor targets are listed first followed by neural targets. Gene function and biological process are as given by FlyBase http://www.flybase.net, March 2004.

From: Prediction and verification of microRNA targets by MovingTargets, a highly adaptable prediction method

Target miRNA # target sites dG of miRNA-mRNA hybrid (kcal/mole) Molecular Function Biological process
CrebA mir-92b
mir-312
3
4
-16, -24, -19
-16, -20, -17, -22
transcription factor salivary gland development
fkh mir-315 3 -18, -16, -17 transcription factor salivary gland morphogenesis
Eip74EF mir-34 3 -25, -27, -21 transcription factor mesoderm development
Eip93F A mir-280 A 3 -21, -15, -16 transcription factor autophagy, induction of apoptosis by hormones
pros mir-34 3 -18, -17, -18 transcription factor ectoderm development
zfh1 mir-5 3 -17, -15, -15 transcription factor ectoderm development, mesoderm development
zfh2 mir-276a-3 3 -16, -15, -17 transcription factor, RNA binding ectoderm development
SoxN mir-34
mir-309
3
3
-23, -24, -16
-19, -16, -20
transcription factor, DNA bending ectoderm development, visual perception
HLHm5 mir-7 3 -24, -24, -26 transcription factor ectoderm development, cell proliferation
CG32527B mir-34 3 -25, -24, -21 multiple (including transcription factor) unknown
ab let-7 5 -20, -25, -17, -17, -19 transcription factor transmission of nerve impulse, sex determination
sbb mir-33 3 -16, -17, -19 transcription factor axon guidance, axon target recognition, larval walking behavior
nerfin-1 mir-279
mir-286
3
3
-19, -17, -24
-19, -16, -27
transcription factor neuronal lineage restriction
Syn mir-92b 3 -19, -20, -15 unknown neurotransmitter secretion, synaptic vesicle exocytois
synaptogyrin mir-313 3 -17, -16, -15 unknown synaptic vesicle exocytosis, regulation of calcium ion dependent exocytosis
Pkc98E mir-210 3 -15, -15, -15 multiple multiple (including neural processes)
nAcRbeta-96A mir-210 3 -19, -18, -23 nicotinic acetylcholine-activated cation-selective channel activity, acetylcholine receptor activity multiple (including neural processes)
W A bantam
mir-280 A
4
3
-21, -16, -27, -17
-17, -19, -15
unknown induction of apoptosis, programmed cell death
kel mir-310
mir-311
mir-312
3
4
4
-20, -19, -22
-17, -21, -19, -15
-16, -18, -20, -17
actin binding apoptosis, ovarian ring canal formation
RhoGEF2 mir-9c 3 -16, -16, -18 Rho guanyl-nucleotide exchange factor activity, diacylglycerol binding multiple
adat mir-3
mir-309
mir-318
3
3
3
-28, -17, -19
-23, -16, -15
-26, -15, -20
tRNA specific adenosine deaminase activity purine base metabolism
bru-2 mir-9a
mir-9c
3
3
-19, -17, -16
-19, -15, -16
RNA binding mRNA processing, protein metabolism
CG32062A mir-12
mir-280 A
4
3
-19, -17, -23, -15
-16, -16, -19
RNA binding unknown
fus mir-303 3 -16, -15, -16 RNA binding epidermal growth factor receptor signaling pathway
mbl A mir-280 A 3 -17, -17, -21 RNA binding, DNA binding mesoderm development
Asph mir-9b 3 -21, -19, -16 peptide-aspartate beta-dioxygenase activity transmembrane receptor protein tyrosine kinase signaling pathway
CG32429 mir-33 3 -17, -17, -18 unknown unknown
lmg mir-34 3 -27, -20, -22 unknown mitotic anaphase
wb mir-34 4 -22, -22, -19, -23 binding, structural molecule activity cell-cell adhesion, cell-matrix adhesion, signal transduction
Cbl mir-34 3 -22, -17, -22 ligase activity cellular defense response
Pdi mir-34
mir-263b
mir-305
mir-316
4
3
4
3
-26, -23, -30, -20
-18, -15, -17
-24, -18, -19, -22
-21, -16, -21
protein disulfide isomerase activity protein folding, protein modification
CG31637 mir-92b 3 -17, -18, -17 sulfotransferase activity carbohydrate metabolism
CG3689 mir-210 3 -20, -15, -17 pre-mRNA splicing factor activity mRNA cleavage, nuclear mRNA splicing, via spliceosome
CG8475 mir-263b 3 -18, -17, -21 kinase activator activity, phosphorylase kinase regulator activity glycogen metabolism
didum mir-276a-3 3 -18, -16, -15 multiple multiple
Ggamma1 mir-277 3 -19, -16, -15 heterotrimeric G-protein GTPase activity G-protein coupled receptor protein signaling pathway
CG1441 mir-278 3 -30, -22, -26 oxidoreductase activity unknown
Rh6 mir-278 3 -19, -22, -20 G-protein coupled photoreceptor activity phototransduction, visual perception, sensory perception
CG31163A mir-289 A 3 -17, -15, -17 SH3/SH2 adaptor protein activity unknown
CG18854 mir-306-3 4 -27, -19, -24, -21 inositol-triphosphate 3-kinase activity unknown
CG7908 mir-309 3 -17, -19, -22 zinc ion binding, metalloendopeptidase avtivity cell surface receptor linked signal transduction, proteolysis and petidolysis
CG14507 mir-276a-5
mir-276b-5
3
3
-24, -20, -21
-24, -20, -21
phospholipase A2 activity unknown
CG33085A,B mir-184-3
mir-284 A
3
3
-21, -22, -16
-23, -25, -17
argininosuccinate lyase activity unknown
CG32316B mir-184-3
mir-278
3
3
-20, -22, -16
-18, -20, -23
oxoglutarate dehydrogenase (succinyl-transferring) activity tricarboxylic acid cycle
CG32912B mir-279 3 -15, -21, -16 peptidoglycan recognition activity immune response
CG33047A,B mir-133
mir-284 A
3
3
-19, -17, -19
-27, -17, -15
alpha-L-fucosidase activity O-glycoside catabolism, fucose metabolism
CG33075B mir-306-3 3 -26, -20, -27 carrier activity transport
CG32956B mir-9a
mir-9b
mir-9c
mir-33
5
5
5
3
-17, -19, -18, -21, -18
-17, -20, -16, -20, -17
-17, -22, -18, -20, -18
-19, -23, -18
multiple multiple
CG33038B mir-9a
mir-9b
mir-9c
4
5
4
-20, -17, -16, -18
-18, -19, -17, -16, -16
-21, -17, -17, -19
multiple heparan sulfate proteoglycan biosynthesis, proton transport
CG32791 mir-31a 3 -15, -17, -15 unknown multiple
tinc mir-34 3 -22, -23, -33 unknown unknown
CG9932 mir-263b 4 -16, -21, -20, -21 unknown unknown
CG30389 mir-14
mir-34
3
3
-17, -16, -25
-28, -21, -24
unknown unknown
CG3975 mir-33 3 -17, -19, -22 unknown unknown
CG8963 mir-6 3 -16, -17, -17 unknown unknown
CG3638 mir-184-3 3 -27, -17, -22 unknown unknown
CG33006B mir-278 3 -20, -15, -23 unknown unknown
CG31305B mir-278 4 -16, -32, -29, -25 multiple unknown
CG32206A mir-289 A 3 -15, -15, -16 unknown unknown
CG12071 mir-305 3 -17, -21, -19 unknown unknown
  1. A: exact 5' and 3' ends for mir-263a, mir-274, mir-280, mir-282, mir-284, mir-289 have not yet been determined (6); target predictions for these microRNAs are likely to change once the precise microRNA sequences are known
  2. B: gene annotated as a "gene cassette"; molecular function and biological process listed is the union of molecular functions and biological processes for each ORF in the putative dicistronic transcript