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Table 2 Comparison of fluorescent signals obtained with S. aureus transcripts hybridized on specific (S. aureus) versus non-specific (E. coli) oligonucleotide probes as a function of target amounts and hybridization temperatures.

From: A generic approach for the design of whole-genome oligoarrays, validated for genomotyping, deletion mapping and gene expression analysis on Staphylococcus aureus

  S. aureus probes E. coli probes
  RCy3/Cy5 Slope RCy3/Cy5 Slope
Temp. 55°C Cy3/Cy5: 5 μg/5 μg 0.95 1.02 0.95 1.2
Temp. 55°C Cy3/Cy5: 10 μg/10 μg 0.99 0.94 0.95 1.2
Temp. 55°C Cy3/Cy5: 5 μg/10 μg 0.86 0.48 0.38 0.39
Temp. 60°C Cy3/Cy5: 5 μg/5 μg 0.98 0.94 0.96 1.78
Temp. 60°C Cy3/Cy5: 10 μg/10 μg 0.98 0.94 0.98 1.25
Temp. 60°C Cy3/Cy5: 5 μg/10 μg 0.85 0.53 0.61 0.72