Table 2 Comparison of fluorescent signals obtained with S. aureus transcripts hybridized on specific (S. aureus) versus non-specific (E. coli) oligonucleotide probes as a function of target amounts and hybridization temperatures.
S. aureus probes | E. coli probes | |||
|---|---|---|---|---|
RCy3/Cy5 | Slope | RCy3/Cy5 | Slope | |
Temp. 55°C Cy3/Cy5: 5 μg/5 μg | 0.95 | 1.02 | 0.95 | 1.2 |
Temp. 55°C Cy3/Cy5: 10 μg/10 μg | 0.99 | 0.94 | 0.95 | 1.2 |
Temp. 55°C Cy3/Cy5: 5 μg/10 μg | 0.86 | 0.48 | 0.38 | 0.39 |
Temp. 60°C Cy3/Cy5: 5 μg/5 μg | 0.98 | 0.94 | 0.96 | 1.78 |
Temp. 60°C Cy3/Cy5: 10 μg/10 μg | 0.98 | 0.94 | 0.98 | 1.25 |
Temp. 60°C Cy3/Cy5: 5 μg/10 μg | 0.85 | 0.53 | 0.61 | 0.72 |