Figure 4
From: Four genomic islands that mark post-1995 pandemic Vibrio parahaemolyticus isolates
A. Schematic representation of VPaI-1 showing arrow heads representing primer pairs used in this study. PCR analysis of the 41 V. parahaemolyticus strains is shown representing the results from 3 primer pairs. Lanes 1 and 44 represent size markers; lanes 2 to 42 contain strains analysed in the same order as in Table 2. For overlapping PCR primer pair analysis only VPaI-1-positive strains were examined, lanes 2 to 25 contain strains analysed in the same order as in Table 3. PCR primer pairs used are indicated under each agarose gel. B. Schematic representation of tRNA-Met site in VPaI-1-negative strains. Arrow heads represent primer pair used, which bind to the 5' and 3' core chromosomal flanking genes. PCR analysis of the 17 VPaI-1-negative isolates (lanes 26 to 42) from section A. The band size is marked on the left side of the PCR image. An expected 2.9 kb product was obtained for all strains except 428/00 and 30824 (Lanes 16 and 17). Lane 1 contains marker. C. Schematic representation of tRNA-Met site in VPaI-1 negative strains 428/00 and 30824. Arrow heads represent primer pair used. Long Template Expand PCR on strains 428/00 and 30824 showed 4 kb of DNA at this insertion site. The band size is marked on the left side of the PCR image.