Direct and heterologous approaches to identify the LET-756/FGF interactome

BMC Genomics

Table 4 Summary of interaction strengths

Interactors	Y2H- screen	Co-IP	Coloc	Deloc	Class
RPS-16	++	++	+	+	I
FTT-1	++	+++	-	+	I
FTT-2	++	+++	-	+	I
KIN-10	++	+	+	+	I
PAL-1	+++	+	+	+	I
C15C6.2	++	++	+	-	II
RPL-6	++	++	-	+	II
DAF-21	+	++	ND		III
COL-129	++	+	+	-	III
SKR-2	++	++	ND		III
KIN-3	+	++	+/-	-	III
C18A3.3	+	++	ND		III

Scores obtained in the different assays used: (i) two-hybrid assays, (ii) co-immunoprecipitation (co-IP), (iii) colocalization by immunofluorescence in single transfected cells (Coloc) and (iv) delocalization (Deloc) of one or the two partners induced by the cotranfection. Interactions were classified as I (the strongest) to III (the weakest).

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