Skip to main content

Table 3 Real-time RT-PCR confirmation of microarray results

From: Gene expression alterations in brains of mice infected with three strains of scrapie

Encoded protein/gene Symbol Microarray fold change RT-PCR fold change* (P value)
   ME7 22L RML Ave. ME7 22L RML Ave.
Diazepan binding inhibitor Dbi 1.2 1.5 1.7 1.5 2.0 (0.021) 2.2 (0.009) 1.9 (0.026) 2.0 (0.001)
CD9 antigen Cd9 1.3 1.9 1.5 1.6 2.9 (0.015) 4.2 (0.001) 3.7 (0.013) 3.6 (0.000)
CathepsinB Ctsb 1.4 1.7 1.3 1.5 1.7 (0.019) 2.0 (0.003) 1.9 (0.009) 1.9 (0.011)
Chemokine (C-X3-C) receptor 1 Cx3cr1 1.2 1.2 1.4 1.3 1.8 (0.107) 3.2 (0.025) 2.0 (0.068) 2.4 (0.011)
Atpase, Na+/K+ transporting, beta 1 Atp1b -1.6 -1.3 -1.2 -1.4 -1.3 (0.185) -1.3 (0.195) -1.3 (0.205) -1.3 (0.02)
Glial fibrillary acidic protein Gfap 4.9 11.7 4.3 6.3 11.6 (0.005) 14.6 (0.003) 12.3 (0.005) 12.8 (0.000)
Apolipoprotein E Apoe 1.9 1.8 2.0 1.9 2.2 (0.005) 2.1 (0.013) 2.7 (0.086) 2.3 (0.002)
Glyceraldehydes-3-phosphate dehydrogenase Gapdh NA NA NA NA -1.1 (0.619) -1.0 (0.798) -1.2 (0.445) -1.1 (0.393)
Actin, beta, cytoplasmic Actb 1.1 1.2 1.0 1.1 1.0 (0.829) 1.3 (0.275) 1.1 (0.693) 1.1 (0.352)
  1. NA, not available, Gapdh not represented on microarrays
  2. *Fold change for each strain was calculated by dividing the average mRNA level from the 2 infected animals by the average of the relative mRNA level of the two control animals.