Figure 2

Oligonucleotides printed on the microarray for the screening of SD6 epitope C-S8c1 FMDV variants. Top: Amino acid and nucleotide sequence encoding the epitope defined by mAb SD6 in FMDV C-S8c1 VP1, between codons 136 and 149. The column on the left shows the names of the oligonucleotides used in this work depicted as colored boxes on the right; the boxes in green represent oligonucleotide sequences identical to the wild type nucleotide sequence written at the top; the boxes in blue represent the oligonucleotides with a sequence corresponding to the different mAb SD6-scape mutants; the boxes in yellow represent oligonucleotide sequences used as negative hybridization controls. Colored, empty boxes include the wild type nucleotide. The mutations are specified in the corresponding position. The enquired position is located at the center of the oligonucleotide. The column on the right gives the predicted Tm value for each oligonucleotide, calculated according to Tm = 69.5 + 0.41 × (X%G+C)-650/total nucleotide number. The origin of the different mutations analysed is given in Methods and in Figure 1.