Figure 3

Scheme of the successive steps from the copying of FMDV genomic RNA to scanning of the microarray. RT-PCR was performed using primers 1R1L (phosphorylated at its 5'-end) and pUL; their sequences are given in Methods. Green boxes indicate those steps for which a number of variables were tested; details on the results of such variable screening will be provided upon request. The final protocol used for the different steps is detailed in Methods.