qRT-PCR of selected genes identified as differentially expressed in A. gambiae abdomens by digital Northern analysis. A. AS 996, a cathepsin B-like protease; B. AS 447, Vitellogenin; C. AS 475, Mucin; D. AS 145, a putative high molecular weight kininogen; and E. AS 99, Chymotrypsin 2. Transcript abundances were normalized against the abundance of RP S7 under the same condition and shown as genomic equivalents * 10,000 for S, RB, and P. berghei-infected (IRB) adult female mosquitoes 30 h post treatment. The arithmetic means of three biological replicates SEM are shown. ANOVA was used to compare the means, and a post hoc Tukey test was used to make pairwise comparisons. Statistically significant differences, P < 0.05 are discussed in the text.